feuA [2017-8-11 16:0:3]

ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein), with [protein|5C52DB31F378E49AABE5D937DD901A68F3810477|YusV] as ATPase

locus: BSU01630

pI: 8.02

mw: 34.95 kDa

protein length: 317 aa

gene length: 951 bp

function: acquisition of iron

product: ABC transporter for the siderophores Fe-enterobactin and Fe-bacillibactin (binding protein) [pubmed|28283524]

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.5|Uptake of iron/ siderophores]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.1|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.4|ABC transporters for the uptake of iron/ siderophores]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 182,370 → 183,323

The protein

Protein family

• Fe/B12 periplasmic-binding domain (according to Swiss-Prot)

[SW|Domains]

• Two independent non-symmetric globular domains, connected by a long alpha-helix (residues 143-164 of the mature protein).

Modification

• FeuA is a lipoprotein with a N-acetyl-S-diacyl-glyceryl-cysteine structure [Pubmed|22303020]

Structure

• [PDB|2PHZ], [PDB|2WI8], [PDB|2WHY] (complex with ferri-bacillibactin), [PDB|2XUZ] (complex with ferri-enterobactin), [PDB|2XV1] (complex with ferric mecam)

[SW|Localization]

• associated to the membrane (lipoprotein) [Pubmed|22303020]
• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|516B13F337FD346B4A4A268E35D1EBABB05957E1|feuA]-[gene|57402D137E3D3791CAF0696421224F4E1DC1BA48|feuB]-[gene|F38F6D4841B3044EA0496B3A1C1D484018BDFEA4|feuC]-[gene|745167427618C4DA71848F9362043B66BC4E392E|ybbA]
  description: [Pubmed|12354229]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17725565], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [pubmed|12354229], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]
  • [protein|EB28A65CECE994DF2DF486DEACF40F2533703DB0|Btr]: activation, in the presence of the co-activators bacillibactin or enterobactin [Pubmed|17725565], in [regulon|EB28A65CECE994DF2DF486DEACF40F2533703DB0|Btr regulon]

  regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
  • induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]) [pubmed|29133393]
  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

  additional information

  • the presence of an iron-responsive element bound by [SW|CitB] between ''[SW|feuA]'' and ''[SW|feuB]'' suggests iron-dependent regulation by [SW|CitB] [Pubmed|10468622]

Other regulations

• [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB]: translation control,

References

• 20086155,19746494,19673474,14563870,16672620,10092453,16889643,17725565,12354229,18957862,18763711,22303020,10468622,28283524